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SHORT COURSE
Imaging Mass Spectrometry of Tissue Sections
Medical Mass Spectrometry Laboratory, Uppsala Biomedical Centre, Uppsala University, Uppsala, Sweden
Saturday June 13th (Seminar, lectures)
Sunday June 14th (Practical, demos and hands-on)
Register here
This course will focus on the analysis of tissue sections using Imaging Mass Spectrometry. It will cover all aspects of the experiment from general study planning, sample collection, matrix deposition, instrumentation to data analysis such classification of the tissue based on its molecular content. Lectures from leading groups in the field will be given on the 13th of June and practical training in sample preparation, imaging mass spectrometry analysis and instrumentation, data analysis will be held on the 14th of June. The course will be held at the Medical Mass Spectrometry Laboratory, Dept. of Pharmaceutical Biosciences, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Course details
Saturday June 13th – Lectures open for all registrants
Sunday June 14th Practical course for a limited number of registrants (maximum 20 persons)
LECTURES ON IMAGING MASS SPECTROMETRY SATURDAY JUNE 13TH
General Study Planning – Review of the general requirements of modern bioanalytical investigation and description of several case studies to demonstrate the role of imaging mass spectrometry.
Sample Collection – A crucial step to successful Imaging Mass Spectrometry analysis. Each step in the process of collection and conservation (dissection, flash-freezing, fixation, embedding, storage) will be reviewed and recommended protocols described.
Sample Preparation – This includes cryo-sectioning, sample stabilization, matrix application, on-tissue digestion, tissue washing, and combined Imaging Mass Spectrometry and histology techniques.
Ionization fundamentals – The choice of ionization technique is crucial to an imaging experiment because it determines which biomolecules can be detected and the sensitivity which they can be analyzed. The lectures provide an overview of the mechanistic background and capabilities of the most common and novel ionization techniques.
Mass Analysis and Instrumentation fundamentals – The lectures include an overview of the mass analyzers commonly used in imaging mass spectrometry, and compare and contrast the characteristics relevant to an experiment and covers sensitivity, accuracy, speed, identification). In context of applications the lecture propose which type of instrument that is suited to solve a particular application.
Data Analysis – This section includes data processing, visualization, classification, storage and access, and links with other data resources.
Complementary Imaging – Here a summary on how to integrate the results from different imaging modalities will be demonstrated (e.g., immunohistochemistry, magnetic resonance imaging, histology) to improve the anatomical/functional/biochemical specification of the biochemical analysis.
Web resources – A summary of available web-resources for imaging mass spectrometry investigations will be provided, including mass spectrometry tools, data analysis tools, databases, and literature guides.
EXPERIMENTAL WORK SUNDAY JUNE 14TH
Sample stabilization, Sample preparation including different matrix deposition instruments,
MALDI imaging using MS and MS/MS on drugs and endogenous compounds, Data analysis using different softwares.
INFORMATION
Contact Dr. Per Andrén (phone +46 – 18 471 7206, email per.andren@bmms.uu.se or Göran Lidgren, the Swedish Academy of Pharmaceutical Sciences (+46 – 8 723 5044, email goran.lidgren@lakemedelsakademin.se) for more information.
REGISTRATION
Click here to register
TUTORS AND LECTURERS
Dr. Per Andrén, Uppsala University
Dr. Garry Corthals, Turku Centre for Biotechnology
Dr. Isabelle Fournier, University of Lille
Dr. Ron Heeren, AMOLF, Amsterdam
Dr. Liam McDonnell, Leiden University
Dr. Michel Salzet, University of Lille
Dr. Markus Stoeckli, Novartis, Basel
Click here to view the program